Thin-layer Chromatography of Lipids
ثبت نشده
چکیده
There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is the quality of the final result that matters and not how it is achieved.
منابع مشابه
Preparative scale separation of neutral lipids and phospholipids by centrifugally accelerated thin-layer chromatography.
Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters.
متن کاملA method for microdetermination of major neutral lipids and an application of the procedure to tissue lipids.
Cholesterol, cholesteryl esters, triglycerides and fatty acids as major neutral lipids and phospholipids were examined in quantitative analysis. The method consisted of three steps: (1) separation of lipids by one-dimensional thin-layer chromatography on silica gel plates; (2) elution of neutral lipids from scraped silica gel with chloroform-methanol (4:1); and (3) colorimetric determination of...
متن کاملTwo dimensional thin-layer chromatography of polar lipids.
A two-dimensional thin-layer chromatographic system that separates all of the known polar lipids of milk and mammary tissue is described. The compositions of phospholipids of milk and of the mammary tissue from which the milk was derived are presented.
متن کاملClinical Diagnosis: Chromatography
See also: II/Chromatography: Paper Chromatography. Chromatography: Gas: Detectors: Selective; Gas Chromatography}Mass Spectrometry. Chromatography: Liquid: Detectors: Mass Spectrometry. Chromatography: Thin-Layer (Planar): Densitometry and Image Analysis; Layers; Mass Spectrometry; Modes of Development: Conventional; Modes of Developement: Forced Flow, Over Pressured Layer Chromatography and Ce...
متن کاملA two-dimensional thin-layer chromatography procedure for simultaneous separation of ceramide and diacylglycerol species.
We have developed a novel, simple, and rapid, two-dimensional thin-layer chromatography method to separate 1,2-, 1,3-diacylglycerols and ceramides containing alpha-hydroxy and normal fatty acids from other neutral lipids on one 10 x 10 cm precoated silica gel plate. The three solvent systems used in succession leave the phospholipids at the origin and separate neutral lipids of interest into co...
متن کاملA compositional based model for the tear film lipid layer.
BACKGROUND The tear film lipid layer is formed from lipids secreted by meibomian glands of the eyelid. After initial analyses of these lipids we concluded that an understanding of the function of the various classes of lipids in a normal lipid layer could only be understood after detailed investigations of both polar and nonpolar lipids of the meibomian gland. METHODS Meibomian gland secretio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011